Quantitative determination of calprotectin in feces

Calprotectin quantitative determination in feces – is a non-invasive biochemical test designed to assess intestinal mucosal inflammation. Calprotectin is a protein found in neutrophil granules, and its levels in feces increase when there is an inflammatory process in the intestinal wall. This test is particularly useful for distinguishing inflammatory bowel diseases (e.g., Crohn's disease, ulcerative colitis) from functional digestive disorders, such as irritable bowel syndrome (IBS).

The test is widely applied in cases of chronic diarrhea, abdominal pain, blood in stools or other unclear intestinal symptoms. The determination of calprotectin levels helps decide if further invasive tests, such as colonoscopy, are needed.

Test principle: The test is performed using an immunoassay method (ELISA) or an immunochromatographic method, allowing quantitative determination of calprotectin concentration in feces. The result is evaluated against established limits – the higher the calprotectin level, the more intense the intestinal inflammation.

Recommendations:

• Recommended for chronic or recurring diarrhea, abdominal pain, blood or mucus in stools, and for monitoring the course and treatment effectiveness of inflammatory bowel diseases.
• The test helps avoid unnecessary invasive procedures for patients whose symptoms suggest functional rather than organic disorders.
• The sample should be collected in a clean plastic container, free from urine or water contamination, preferably delivered to the laboratory on the same day or stored in a refrigerator (according to laboratory instructions).
• Result interpretation requires evaluation of the overall clinical picture – calprotectin levels can also increase due to infections or certain medications (e.g., NSAIDs).
• Repeating the test can be useful for monitoring treatment progress or changes in symptoms.