Determination of Leflunomide Concentration

Leflunomide is an immunosuppressive drug most commonly used for the treatment of rheumatoid arthritis and other autoimmune diseases. Determining the concentration of the drug in the blood is important to ensure therapeutic effectiveness and avoid toxic reactions.

The test is designed to determine the concentration of Leflunomide in the patient's blood serum. This helps doctors tailor the dosage to the individual needs and treatment response of the patient, as well as monitor potential side effects caused by the drug.

The method used to determine the concentration of Leflunomide is liquid chromatography and mass spectrometry (LC-MS/MS). This technique allows for accurate and sensitive measurement of the drug concentration in the blood.

Procedure:

1. Sample Collection: a blood sample is taken from a vein.
2. Preparation: blood serum is separated from other blood components by centrifugation.
3. Analysis: the prepared serum is analyzed using LC-MS/MS equipment.
4. Interpretation of Results: the obtained data is interpreted according to established therapeutic and toxic concentration limits of Leflunomide.

Results: The test results are presented as the concentration of Leflunomide in the blood (ng/ml). These data help determine whether the drug concentration is within the therapeutic range or if the dosage needs to be adjusted.

Indications:

• Monitor the therapeutic effectiveness and safety of Leflunomide.
• Evaluate individual differences in drug metabolism.
• Determine the risk of adverse reactions caused by the drug.

The test duration is 3 weeks.